ISSN: 2148-8274 / E-ISSN: 2587-0084
, Türk Üreme Tıbbı ve Cerrahisi
Dergisi

Turkish Journal of Reproductive Medicine and Surgery

Dernek Sitesi
Kayıtlı İndexler
ORİJİNAL ARAŞTIRMALAR

Şiddetli Oligoastenoteratozoospermisi Olan Olgularda, Kalsiyum İyonofor İle Oosit Aktivasyonu İşleminin, Embriyogenez ve Klinik Sonuçlara Etkilerinin Araştırılması
Investigation of the Effects of Calcium Ionophore Oocyte Activation on Embryogenesis and Clinical Outcomes in Cases with Severe Oligoastenotherazoospermia
Received Date : 11 Jan 2022
Accepted Date : 16 Feb 2022
Available Online : 23 Feb 2022
Doi: 10.24074/tjrms.2022-88104 - Makale Dili: TR
TJRMS. 2021;5(3):102-8
ÖZET
Amaç: Bu araştırma kapsamında, oosit aktivasyon bozukluğu yaşayan şiddetli oligoastenoteratozoospermi (OAT) hastalarının intrasitoplazmik sperm injeksiyonu (ICSI) işlemi sonucunda, kalsiyum iyonofor işlemi uygulanan ve uygulanmayan olguların embriyo kalitesi ve klinik gebelik sonuçları retrospektif olarak incelenmiş ve literatüre katkı sağlaması amaçlanmıştır. Gereç ve Yöntemler: Ocak 2014 ile Aralık 2019 tarihleri arasında Centrum Klinik Kadın Sağlığı Merkez’ine başvuruda bulanan 100 ICSI olgusunun verilerinin retrospektif veri tarama çalışmasıdır. Analize dahil edilme kriterleri şiddetli OAT tanısı alan ve bu nedenle erkek faktörü infertilitesi olarak değerlendirilmiş olgulardır. ICSI sonrası oosit aktivasyonu için kalsiyum iyonofor uygulananlar deney grubu olarak, uygulanmayalar ise kontrol grubu olarak çalışmaya dahil edilmiştir. Her iki grup arasında toplam oosit sayısı, 2 pn fertilize oosit sayıları, fertilizasyon yüzdesi, 3. gün toplam embriyo sayıları, iyi kalite klivaj embriyo sayıları, 5. gün toplam embriyo sayıları, iyi kalite blastosist sayıları, klinik gebelik yüzdesi, biyokimyasal gebelik ve abortus yüzdesi sonuçları karşılaştırılmıştır. Bulgular: Şiddetli OAT olgularında kalsiyum iyonofor kullanılan yani deney grubunun fertilize oosit sayılarında, fertilizasyon yüzdelerinde, iyi kalite klivaj embriyo sayıları, iyi kalite blastosist sayılarında istatistiksel açıdan anlamlı bir artışın olduğu gözlemlenmiştir. Klinik olarak karşılaştırılması yapılan klinik gebelik ve canlı doğum oranlarında yüzde olarak deney grubunda bir artış görülmesine karşın istatiksel olarak anlamlı bir artışın olmadığı gözlemlenmiştir. Sonuç: Klinik açıdan daha iyi fikir sahibi olunabilmesi için daha kapsamlı çalışmalara ihtiyaç duyulmaktadır.
ABSTRACT
Objective: In this study, after intracytoplasmic sperm injection (ICSI) was performed to patients with severe oligoastenoteratozoospermia (OAT) in oocyte activation disorder, the embryo quality and clinical pregnancy outcomes of the cases with or without calcium ionophore treatment were retrospectively examined and contribute to the literature. Materials and Methods: This is a retrospective data scanning study of the data of 100 ICSI cases who applied to Centrum Clinical Women’s Health Center between January 2014 and December 2019. Inclusion criteria in the analysis were cases diagnosed with severe OAT and therefore evaluated as male factor infertility. Those who were applied calcium ionophore for oocyte activation after ICSI were included in the study as the experimental group, and those who were not applied as the control group. Between both groups, total oocyte count, 2 pn fertilized oocyte count, fertilization percentage, total embryo counts on day 3, good quality cleavage embryo counts, day 5 total embryo counts, good quality blastocyst counts, clinical pregnancy percentage, biochemical pregnancy and abortus. percentage results were compared. Results: It was observed that there was a statistically significant increase in the number of fertilized oocytes, fertilization percentage, good quality cleavage embryo numbers, and good quality blastocysts in the experimental group, which was calcium ionophore treated severe OAT cases. Although there was an increase in the clinical pregnancy and live birth rates as a percentage in the experimental group, which were compared clinically, it was observed that there was no statistically significant increase. Conclusion: More comprehensive studies are needed to have a better clinical opinion.
REFERANSLAR
  1. Mahutte NG, Arici A. Failed fertilization: is it predictable? Curr Opin Obstet Gynecol. 2003;15(3):211-8. [Crossref]  [PubMed] 
  2. Bakı Acar D, Bastan A. Activation of bovine oocytes following ICSI and effect of activation on embryo according to developmental stages. Kafkas Univ Vet Fak Derg. 2011;17(4):631-6. [Crossref] 
  3. Montag M, Köster M, van der Ven K, Bohlen U, van der Ven H. The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle. Reprod Biomed Online. 2012;24(5):521-6. [Crossref]  [PubMed] 
  4. Ebner T, Montag M; Oocyte Activation Study Group, Montag M, Van der Ven K, Van der Ven H, Ebner T, Shebl O, Oppelt P, et al. Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online. 2015;30(4):359-65. [Crossref]  [PubMed] 
  5. Ahmady A, Michael E. Successful pregnancy and delivery following intracytoplasmic injection of frozen-thawed nonviable testicular sperm and oocyte activation with calcium ionophore. J Androl. 2007;28(1):13-4. [Crossref]  [PubMed] 
  6. Rybouchkin AV, Van der Straeten F, Quatacker J, De Sutter P, Dhont M. Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity. Fertil Steril. 1997;68(6):1144-7. [Crossref] 
  7. Field, Andy ve Graham Hole. Deneyler nasıl tasarlanır ve raporlanır . Sage, London Thousand Oaks New Delhi. 2002.
  8. Tejera A, Mollá M, Muriel L, Remohí J, Pellicer A, De Pablo JL. Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient. Fertil Steril. 2008;90(4):1202.e1-5. [Crossref]  [PubMed] 
  9. Ramadan WM, Kashir J, Jones C, Coward K. Oocyte activation and phospholipase C zeta (PLCζ): diagnostic and therapeutic implications for assisted reproductive technology. Cell Commun Signal. 2012;10(1):12. [Crossref]  [PubMed]  [PMC] 
  10. Yanagida K. Complete fertilization failure in ICSI. Hum Cell. 2004;17(4):187-93. [Crossref]  [PubMed] 
  11. Montag M, Köster M, van der Ven K, Bohlen U, van der Ven H. The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle. Reprod Biomed Online. 2012;24(5):521-6. [Crossref]  [PubMed] 
  12. Hoshi K, Yanagida K, Yazawa H, Katayose H, Sato A. Intracytoplasmic sperm injection using immobilized or motile human spermatozoon. Fertil Steril. 1995;63(6):1241-5. [Crossref] 
  13. Heindryckx B, Van der Elst J, De Sutter P, Dhont M. Treatment option for sperm- or oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI. Hum Reprod. 2005;20(8):2237-41. [Crossref]  [PubMed] 
  14. Yamano S, Nakagawa K, Nakasaka H, Aono T. Fertilization failure and oocyte activation. J Med Invest. 2000;47(1-2):1-8.
  15. Long R, Wang M, Yang QY, Hu SQ, Zhu LX, Jin L. Risk of birth defects in children conceived by artificial oocyte activation and intracytoplasmic sperm injection: a meta-analysis. Reprod Biol Endocrinol. 2020;18(1):123. [Crossref]  [PubMed]  [PMC] 
  16. Chan C, Ryu M, Zwingerman R. Preimplantation genetic testing for aneuploidy: A Canadian Fertility and Andrology Society Guideline. Reprod Biomed Online. 2021;42(1):105-16. [Crossref]  [PubMed] 
  17. Mazzilli R, Cimadomo D, Vaiarelli A, Capalbo A, Dovere L, Alviggi E, et al. Effect of the male factor on the clinical outcome of intracytoplasmic sperm injection combined with preimplantation aneuploidy testing: observational longitudinal cohort study of 1,219 consecutive cycles. Fertil Steril. 2017;108(6):961-72.e3. [Crossref]  [PubMed] 
  18. Capalbo A, Rienzi L, Cimadomo D, Maggiulli R, Elliott T, Wright G, et al. Correlation between standard blastocyst morphology, euploidy and implantation: an observational study in two centers involving 956 screened blastocysts. Hum Reprod. 2014;29(6):1173-81. [Crossref]  [PubMed]